LOCUS       40924_5364       12546 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB5nGGW DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 12546)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1696
                     /codon_start=1
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     protein_bind    1709..1833
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1886..1916
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1970..2626
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHSCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2971..3273
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3317..3441)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3467..3719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3753..3769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3777..3793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3801..3831)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3846..3867)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3903..4155)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4309..5331)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5383..5562)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6068..6092)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6613..7401
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7650..8238
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8424..8564)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8908..9102)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9171..10241)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10673..11299)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1681 ccgccacaac atcgaggacg ctgttatcac aagtttgtac aaaaaagctg aacgagaaac
     1741 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     1801 atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggcct cgagccctcg
     1861 aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     1921 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1981 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2041 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     2101 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     2161 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     2221 atgggatagt gttcactctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     2281 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     2341 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     2401 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     2461 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     2521 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat
     2581 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc
     2641 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     2701 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt
     2761 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     2821 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     2881 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     2941 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga
     3001 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     3061 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     3121 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     3181 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     3241 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     3301 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     3361 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     3421 agctttcttg tacaaagtgg tgataaccta gagctcgaat ttccccgatc gttcaaacat
     3481 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3541 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3601 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3661 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3721 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3841 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc
     3901 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     3961 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     4021 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     4081 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     4141 aatgtttgaa cgatcgggga aattcggggg atcgatcccg cgaactgtgg acgagaactg
     4201 tgccaccaag cgtaaggccg ttctctcgca tttgccttgc taggctcgcg cgagttgctg
     4261 gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat ctactctatt cctttgccct
     4321 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     4381 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     4441 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     4501 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     4561 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     4621 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     4681 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     4741 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     4801 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     4861 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     4921 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     4981 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     5041 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaatgccc caatgtcaag
     5101 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     5161 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     5221 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     5281 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcttattg
     5341 ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg
     5401 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     5461 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     5521 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     5581 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     5641 tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     5701 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     5761 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     5821 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     5881 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     5941 tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta
     6001 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     6061 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     6121 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     6181 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     6241 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     6301 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     6361 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     6421 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     6481 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     6541 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     6601 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     6661 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     6721 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     6781 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     6841 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     6901 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     6961 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     7021 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     7081 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     7141 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     7201 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     7261 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     7321 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     7381 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     7441 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     7501 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     7561 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8341 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8401 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     8461 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8521 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     8581 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8641 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8701 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     8761 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     8821 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     8881 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     8941 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     9001 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     9061 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     9121 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     9181 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     9241 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     9301 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     9361 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     9421 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     9481 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     9541 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     9601 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     9661 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     9721 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     9781 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     9841 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     9901 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     9961 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
    10021 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
    10081 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
    10141 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
    10201 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
    10261 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
    10321 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
    10381 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
    10441 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
    10501 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
    10561 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    10621 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    10681 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    10741 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    10801 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
    10861 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
    10921 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
    10981 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
    11041 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
    11101 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
    11161 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    11221 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    11281 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    11341 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    11401 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    11461 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    11521 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    11581 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    11641 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    11701 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    11761 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    11821 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    11881 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    11941 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    12001 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    12061 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    12121 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    12181 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    12241 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    12301 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    12361 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    12421 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    12481 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    12541 aaacct
//
